Nanowires, Molecular Electronics, and Nanodevices, Ed.
Semester 1 SMBB This course encompasses the basic principles and techniques involved in molecular biology which will enable students to apply these techniques in the genetic engineering laboratory.
The introductory lecture will expose students to genetic engineering and its application in various sectors of the industry such as agriculture, medical, pharmaceutical, environmental, etc. The following lectures will revolve around techniques in genetic engineering, cloning of heterologous genes in the Escherichia coli host which will include common procedures in molecular biology, enzymes important in molecular biology, plasmids and bacteriophage as cloning vectors, gene libraries preparation and screening for gene of interest.
Before semester ends, students are to produce a group poster relevant to genetic engineering as a form of teamworking experience. Peer group evaluation is mandatory.
Some of the concepts taught will be applied in this laboratory practical.
Students will have the opportunity to hands on the isolation of genomic DNA, agarose gel electrophoresis, PCR amplification, DNA ligation, preparation of competent cells, transformation, and lastly verify the clones of interest by plasmid DNA extraction and DNA restriction digestion.D. Yoo, Y.
Min, G. H. Ahn, D.-H. Lien, J. Jang, H. Jeong, Y.
Song, S. Chung, A. Javey, T. Lee, "Highly reliable superhydrophobic protection for organic. The common metals are not affected by anhydrous ammonia. But, even if there is a little moisture, ammonia will react rapidly with copper, brass, zinc and many alloys, especially those containing.
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